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Explain the absence of isolated colonies|Microbiology: Interpretation Of Results - Microchem

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The fluorescence was an indication that the bacteria survived and have taken the plasmid and therefore because we had two isolated colonies, we performed the experiment efficiently.  The phenotype of the colonies is evidence that they were transformed efficiently.Then, drag the loop through the second quadrant once, and zig-zag streak the third quadrant of your plate from the edge inward.Repeat for your fourth quadrant.The results, and the additional cost associated with performing the confirmations are therefore separated.

The plates must incubate at 30 degrees and if the temperature is even a few degrees off the bacteria will be unable to fluoresce.This is because the bacteria that took up the plasmid were able to withstand ampicillin.certainly a contamination and if there are colonies on streak line.

Since a representative portion of a sample is analysed, the results indicate that no growth was obtained in the sample portion tested.A result of ‘no growth’ or ‘zero’ is therefore not scientifically accurate because the sample dilution is not reported, or taken into account.This solution then must undergo a heat shock in water at 42 degrees to create a draft and sweep the plasmids into the pores of the bacterial membrane.Unauthorized use and/or duplication of this material without express and written permission from this Florida Institute of Technology is strictly prohibited.

Love you guys.The plates must incubate at 30 degrees and if the temperature is even a few degrees off the bacteria will be unable to fluoresce.The transformation of bacterial cells is a useful experiment to help develop an understanding of transformation by plasmid DNA.



Question Completion Status: QUESTION 3 Arial How W ...

line and only it shows growth in b/w the streak line then it is.Quantitative analysisAnalysis where a microbial count is determined.The counts are TNTC (Too Numerous to Count) and therefore are reported as more than (>) the upper countable range of the test.

Qualitative analysisAnalysis where the presense or absence of an organism is determined.coli and pVIB plasmids that are transferred to the various plates.Preliminary results can be provided upon request of the client.

When you have a single colony isolated from the rest of the colonies on the plate, it is a pure colony “grown up” from a single bacterium (that wasfrom the rest of the sample, streaked out on the plate).It is essentially a dilution technique that involves spreading a loopful of culture over the surface of an agar plate.

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I totally understand this now.If the amount transferred is too much or too little the transformation may not even occur.Since a representative portion of a sample is analysed, the results indicate that no growth was obtained in the sample portion tested.A result of ‘no growth’ or ‘zero’ is therefore not scientifically accurate because the sample dilution is not reported, or taken into account.

This data from the experiment shows us that the process of transformation by plasmids is very effective.Ampicillin is an antibiotic that is known to treat bacterial infections, in this experiment ampicillin’s role is to kill all bacteria that did not undergo transformation.The fluorescence appearance shows that the bacteria took up the plasmid.

Results may not be reportable for a number of reasons.



If an inoculated plate had colonies between the streak ...

Qualitative analysisAnalysis where the presense or absence of an organism is determined.The LB/Amp+plasmid showed isolated colonies because only the bacteria that took up the plasmid glowed.If the amount transferred is too much or too little the transformation may not even occur.

Also, because there was no ampicillin present there was a major growth of bacteria because the bacteria was replicating without being destroyed by other factors.100 μL cell suspension / 510 μL total suspension.Certain reference methods stipulate that samples with presumptive positive results require further confirmation.

I think that there are various factors that influence transformation efficiency.As predicted the LB/Amp-plasmid showed no growth due to the fact that the ampicillin killed the bacterial cells and there was no plasmid present for resistance.

Hi Kerney! Thanks for showing how to use this tool.After preparing two bacterial cells, one with plasmid and one without, another agar plate is prepared where ampicilin is introduced.help get a clear identification of the bacteria.

certainly a contamination and if there are colonies on streak line.contamination but there can be a chance that colony appears due to.The results on the COA will read as follows for the following counts:Average CFU count = 10 – Results = 10 EAverage CFU count = 350 – Results = 350 E(For ease of interpretation, no dilution factor has been included in the example above).

It depends, if there is no growth or colony appearance on streak.The LB/Amp-plasmid showed no growth due to the fact that the ampicillin killed the bacteria.The fluorescence was an indication that the bacteria survived and have taken the plasmid and therefore because we had two isolated colonies, we performed the experiment efficiently.  The phenotype of the colonies is evidence that they were transformed efficiently.What explanations could be given for the - Answerscom.

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